FIG. 2.

Regulated expression of T-cell and neutrophil chemoattractants in T84 epithelial cells infected with Campylobacter jejuni. RT-PCR analysis of the T-cell chemoattractants CXCL9, CXCL10, and CXCL11 (A), or the neutrophil chemokines CXCL1, CXCL5, and CXCL8 (B) from total RNA isolated from T84 epithelial cells inoculated with C. jejuni strain 81-176 at an MOI of 25:1, 50:1, or 100:1. RNA from uninfected cells (Ctrl) or cells treated with 20 ng/ml TNF-α were used as negative and positive controls, respectively. Reactions performed in the absence of RNA (No RNA) as a negative control. GAPDH verified equal loading between samples. Data are representative of three independent experiments.