FIG. 6.

Campylobacter jejuni flagellin does not stimulate chemokine secretion by T84 epithelial cells. A. C. jejuni and S. enterica serovar Typhimurium flagellins were purified and quantified as outlined in Materials and Methods. Equal amounts of flagellin protein were size separated by SDS-PAGE and stained using Coomassie brilliant blue to verify size and purity. Flagellins of C. jejuni, FlaA (59 kDa), and S. enterica serovar Typhimurium, FliC (50 kDa), are shown. Markers (M) are as indicated. T84 cell monolayers were stimulated with increasing concentrations of flagellin (1, 10, 100, or 1,000 ng/ml) from C. jejuni or S. enterica serovar Typhimurium. Separate monolayers were left untreated or were treated with TNF-α (20 ng/ml) as negative or positive controls, respectively. CCL20 (B) and CXCL8 (C) secretion was measured 12 h after addition of flagellin. Values are means ± standard deviations from a representative experiment (n = 3).