TABLE 3.

CCL20 and CXCL8 secretion and invasion in T84 cells infected with clinical isolates of Campylobacter jejuni

C. jejuni strain	Genotypea			Motilityb	% Invasionc	Chemokine secretion (ng/ml)d
	flaA	cadF	cdtB			CCL20	CXCL8
81-176	+	+	+	+	3.10 ± 0.53	3.36 ± 0.9*	0.57 ± 0.15*
11168	+	+	+	+	0.05 ± 0.04	1.36 ± 0.3*	0.28 ± 0.08*
45-05	+	+	+	+	0.02 ± 0.01	1.16 ± 0.2*	0.26 ± 0.05*
38-73	+	+	+	+	0.05 ± 0.03	1.39 ± 0.1*	0.23 ± 0.06
46-47	+	+	+	+	0.37 ± 0.09	3.22 ± 0.4*	0.54 ± 0.17*
46-49	+	+	+	+	0.12 ± 0.01	0.95 ± 0.3	0.24 ± 0.06*
39-02	+	+	+	+	0.27 ± 0.06	1.22 ± 0.3*	0.26 ± 0.09*
41-70	+	+	+	+	0.04 ± 0.01	0.87 ± 0.1	0.27 ± 0.06*

^aE. coli DH5α and Salmonella 14028s assessed as negative controls did not possess the flaA, cadF, and cdtB genes. Data for genotype analysis are representative of two independently completed experiments.

^bEach of the Campylobacter isolates and Salmonella 14028s assessed as a positive control, were motile. E. coli DH5α assessed as a negative control was nonmotile. Data for motility assays are representative of two independently completed assays.

^cInvasion was assessed as a percentage of the initial inoculum as defined in Materials and Methods. Values are the means ± SD from two to six independent experiments.

^dSecretion of CCL20 or CXCL8 was assessed by ELISA as defined in the Material and Methods section. Values for chemokine secretion are means ± SEM from two to six independently completed experiments. Background levels of CCL20 and CXCL8 in uninfected T84 cells were 0.79 ± 0.26 and 0.13 ± 0.07, respectively. An asterisk denotes a statistically significant difference in chemokine secretion between C. jejuni isolates and uninfected T84 cells.