FIG. 4.
Decreased expression of OMPs in tick cells infected with the St. Maries strain of A. marginale (iIDE8) compared to infected erythrocytes. A total of 7.3 × 106 organisms were loaded in the infected erythrocyte (iRBC) and the 1× iIDE8 lanes. A total of 7.3 × 107 (10×) to 1.6 × 108 (25×) organisms were loaded in the remaining IDE8 lanes. The noninfected IDE8 cells (nIDE8) were used as a negative control, corresponding to the maximum load of iIDE8. Polyclonal, monospecific mouse sera were used to detect OMP1 and OMP8; monoclonal antibody 121/1055 was used to detect OMP9; and monoclonal antibodies 121/161 and 122/255 were used to detect OMP7 and OMP4, respectively.