. 2006 Jun;74(6):3471–3479. doi: 10.1128/IAI.01843-05

TABLE 1.

Primers used for omp transcriptional analysis and sequencing

Gene	Primer^a	Sequence (5′-3′)	Amplicon size (nt)^b	Gene	Primer^a	Sequence (5′-3′)	Amplicon size (nt)^b
omp1	RT/S F	AAGTACGAGTCTGATACCGCACTCAAAC	778		S R	AACAACAATAGAGCGCTACG
	RT/S R	AAAACAGCAGGGTGGCACCG			Q F	TGCCCGAGCACCGAGATTTCTTAT
	Q F	CAGAAATTCCCCGTAGATACCAA			Q R	ATGACAGGGTACCTTTCTGCACCA
	Q R	TGGTCACTTCCTTATTCTCAGCG			Probe	ACTGGAAGATGCGCTGCTTACCGCGA
	Probe	TTGCTACAAGGCCGCAGGAGCC		omp9	RT F	AGCTGGGGCTCTTGCGTTTG
omp2	RT F	GATTGAGTCTATGGGGGAACAAAAGAG	567		RT R	AACATATTCACTATAATCTGACGCTGC	1,096
	RT R	GAGCTTCGAATGGTGCAGAGAG			S F	AGCGTCTACTGATTGTGTTC	1,186
	S F	AATCAGGTTCGGATTCTAGG	1,102		S R	TTCACTATAATCTGACGCTG
	S R	CAGTGAGTCTTCACATGGAG			Q F	GAAGTCACTACACGACCTGACTGT
omp3	RT F	GTGCCTCATTCGTACCTCCTACC	314		Q R	TAAAGCATCTTCGCGGGTCGT
	RT R	GGGGTAAGTAGAGAGGCACTGATG			Probe	TATTCAGTGCGCTGAACACTGCGATCCA
	S F	TCTCCAAATTCCCGGTGCCT	675	omp10	RT F	TCCTTCGGGTTGCTGCGTTG	969
	S R	GGTGGGTGTCCTAGAATCCG			RT R	GCTTACCCCCATTCCAGCAC
omp4	RTF	TTGAGAAACTTGCTCCAATGTTAAAACC	922		S F	CACATTTTGGTTGCATTTATCG	1,146
	RTR	AGCGGAATTGGCACTGTGC			S R	ATTCGCAGTTCTATGCAGCA
	S F	AATTTTTGTGGTTGGCGGTG	1,294		QF	TGGAAGAAGGTAAGAGGCTTGGCA
	S R	CACTCTGCCAACTAAAATAAAAGGA			QR	TCGCCGACCCAACATAGTTAAGCA
	QF	AGCTGGGCATAGGAAGAGAAGCAT			Probe	AAGGCAGAAGCAGAAGCGCGAAGAT
	QR	TGTGTCACCTCCTGTGTGTTGGAA		omp11	RT F	ATGAGCTTTGTAAGGTTTCTTGCC	624
	Probe	TGCACGCGCAAGATTTCGGATAAGCA			RT R	AAGCACCGAGGAGAGCTGTACC
omp5	RT F	AACTCGAGCTTCAGCCCCAG	868		S F	CTACGGGAGTAAATACTTGG	1,145
	RT R	TGCCCTTGAGCCACACTCAC			S R	TACCACATACACGGCAAAAC
	S F	AAGCGTGCGTAGCTAAAACT	1,177		QF	GTGCTTGCTAGTTTGTGCAGGGAT
	S R	TGAAGCTGGTAGAATCCCGG			QR	CAATCTTCGCCTGGTAAGAAACCC
omp6	RT F	CTCCAATCGGAGGGGTTGTG	492		Probe	TGCCATATGTGTGTGCTGGGTTTGGT
	RT R	GCATAAATCCAGTTTAGCCTCC		omp12	RT F	ATGGGATCTATGATGAGGGC	703
	S F	CATCGTGAATATACTGAGGA	694		RT R	CAATAAATAGCGTCACACCCC
	S R	AAACATGGATCACAGACGTT			S F	TGTGAGAGTTGCCGCTTCCG	959
omp7	RT F	GTGGTTAGATCTTTTCTGTTGGG	399		S R	TGCGGTGAGCACGTAGCAAA
	RT R	CGCTCTACCACTGACCTTCATG		omp13	RT F	ATGGTTAAAGCAGGGGCAGCATG	474
	S F	TGGGGAGGGGATGTGTGTTG	1,105		RT R	GCTCCTCGCAACCTTAAATTCC
	S R	CGCGGCACTGCTCTTTATAC			S F	CGTTTTACAACCTTGACTTT	1,156
	Q F	TTCGTGCGCTGTCCTCTTCTACTC			S R	GAAAATACGCGCATACACAC
	Q R	TGTCCGTGCACCAATCTCACTAGA		omp14	RT F	GAATCCGAACCTGATTCCTAGTTCT	690
	Probe	AGTTGCATAATCTTGCGGATGCGCTA			RT R	ACAAGGAGTTGTCCAAGCCGG
omp8	RT F	AACTGGTAGGCTGGAGTTCG	213		S F	TAGTGCCGGGAACCGCAAGT	1,155
	RT R	AGTGATAAGAAATCTCGGTGCTC			S R	GGGATGAACGGTCGTGAAGA
	S F	AGTGAATATGTTGAGAGGGA	1,374

RT, forward (F) and reverse (R) primers used for detection of transcript after reverse transcription; S, forward (F) and reverse (R) primers used to amplify open reading frames for cloning and sequencing; Q, forward (F) and reverse (R) primers used for quantitative PCR; Probe, TaqMan probes used for quantitative PCR.

nt, nucleotides.