TABLE 2.
Peptides used to generate antibodies that reacted with the peptide in an ELISA
| Peptide | Location of peptide (aa)d | Sequence |
|---|---|---|
| OMP1-P1a | 151-160 | CTKKYKDNPERAYR |
| OMP1-P2a | 70-79 | CKEKQQGGTAK |
| OMP1-P3b | 279-287 | CTESPKGSQG |
| OMP2-P1a | 7-19 | CEQKRGRCESAR |
| OMP3-P1a | 35-50 | CITRHPSPTYHHSSPHR |
| OMP3-P2c | 141-155 | CATIYNHPMLSSQPHK |
| OMP3-P3c | 17-26 | CQCHHLCTTNT |
| OMP4-P1b | 176-190 | CSDTIESELFQHTGGD |
| OMP5-P1a | 188-100 | CQGGLSIDSSTSTA |
| OMP5-P3 | 45-53 | CGHKGAGTRR |
| OMP5-P2a | 224-245 | CSSEDRLAAAK |
| OMP6-P1 | 21-30 | CGTGSSAAEAF |
| OMP6-P2a | 131-139 | CGRHWKQGNS |
| OMP7-P1b | 198-209 | CDLKHVGASSVD |
| OMP8-P1b | 172-185 | CALPEHRDFLSLEDA |
| OMP9-P1b | 207-220 | CGTTREDALAATQIV |
| OMP10-P1 | 192-200 | CEEGKRLGNL |
| OMP10-P2 | 268-276 | CSDKDEARRA |
| OMP10-P3 | 182-190 | CSTNAGDGKS |
| OMP11-P1b | 149-168 | CHDEGVVGDLYASE |
| OMP12-P1 | 149-158 | CNIALVRAQT |
| OMP12-P2 | 6-16 | CRATKKGSISVR |
| OMP12-P3 | 39-48 | CRKFRSQGRAY |
| OMP13-P1 | 199-210 | CNAAGAGSSAGQQ |
| OMP13-P3a | 317-325 | CGASSRTRDD |
| OMP14-P1a | 239-249 | CQNTQESKRDEA |
| OMP14-P2a | 378-388 | CLGKTKEKVSAS |
| OMP14-P3a | 36-46 | CASSHGMNGRED |
a
Peptides that elicited antibodies that reacted with the recombinant protein.
b
Peptides that elicited antibodies that reacted with the recombinant protein and A. marginale proteins in erythrocytes and/or IDE8 cells.
c
Peptides that elicited antisera that did not react with A. marginale-infected erythrocytes and/or IDE8 cells but were not tested against the recombinant protein. The remaining peptides elicited antisera that reacted with the peptide in an ELISA but did not react with recombinant proteins.
d
aa, amino acids.