Skip to main content
. 2000 Feb;11(2):555–565. doi: 10.1091/mbc.11.2.555

Figure 4.

Figure 4

Transactivation through the GCCG motif. (A) A luciferase reporter assay was performed using R mutant mink lung epithelial cells. Reporter genes with various numbers of the GCCG motif were transfected into the cells in the absence or presence of BMP stimulation. Luciferase activity was normalized against the cotransfected sea pansy luciferase activity. Experiments were done in duplicate, and the SDs are shown in vertical lines. The values represent fold induction as determined by comparing with the basal 90COLXLUC activity. The fold induction of each reporter activity upon BMP stimulation is shown in the inset. (B) P19 mouse embryonal carcinoma cells were used in a luciferase assay. The fold induction of each reporter activity upon BMP stimulation is shown in the inset. Note the difference of the scale between A and B.