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GFP-Pde1c neurons in the superficial dorsal horn are not immunoreactive for calbindin or calretinin. (A) and (B) show merged images of GFP-immunoreactivity (green) and calbindin-, or calretinin-immunoreactivity (red). (A) and (B) are overlays of single images taken using a wide-field fluorescence microscope. Scale bar, 100 μm in each panel. Dorsal uppermost, medial left both panels.
