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Multiplexed CelF azo-CMC screening plate. In vitro expressed protein from multiplexed plasmid preparation was spotted onto agar plate containing azo-CMC (pH 5.8). Wells are identified by the row (letter) and column (number) on the azo-CMC plate. B11, C12, G2, G11 and H12 showed greatest activity and were selected for separation into individual clones (see Figures 6 and 7).
