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Diameter of cleared zone plotted as a function of increasing concentration of an individual improved CelF mutant. One, 3.0, 5.0 and 10.0 μL of a 27.7 ng/μL solution of F9MP mutant (27.7, 83.1, 139 and 277 ng, respectively) were spotted on an azo-CMC plate at pH 5.8. The resultant cleared zones are pictured in the inset above the graph (incubation at 10 hours, 37°C).
