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Detection of activity of an individual CelF mutant in a multiplexed mixture as number of colonies multiplexed per well increases. Activity contributed by an individual CelF mutant (F9MP) is plotted as a function of number of background colonies multiplexed as described in Methods. The cleared zones on the azo-CMC plate at pH 5.8 are shown in inset at bottom (10 μL spotted; incubation at 10 hours, 37°C).
