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Verification of deglycosylation of de-2H6 by and MALDI-TOF-MS and SDS-PAGE. Panel A. SDS-PAGE analysis of 2H6 and de-2H6. Samples were size fractionated under denaturing conditions on a 3–8% Tris-Acetate Gel and stained with Coomassie blue. Note the lower apparent molecular weight for the deglycosylated heavy chain doublet. Panel B. MALDI-TOF-MS analysis revealed the expected 2% reduction in molecular weight after removal of N-linked glycans in the de-2H6 antibody
