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. 2006 May 4;3:8. doi: 10.1186/1476-9255-3-8

Figure 2.

Figure 2

Inhibition by PP2 or SKI-1 of zymosan- and S.aureus- induced phosphorylation of MAP kinases. Macrophages were pretreated for 15 min with PP2 (1–10 μM), followed by stimulation with zymosan (A) or S.aureus (B) for 20 min. (C) Macrophages were pretreated for 15 min with SKI-1 (5 μM), followed by stimulation with zymosan or S.aureus for 20 min. Equal amounts of cell lysate were run on 10% polyacrylamide gels and probed with phosphospecific antibodies against ERK, p38 and JNK. The membrane was reprobed with ERK-2 antibody to verify equal loading of protein.