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. 2006 Jun 15;116(7):2033–2043. doi: 10.1172/JCI27544

Figure 3. Effect of different neutrophil agonists on the phosphorylation of Ser345.

Figure 3

Neutrophils (1 × 107 cells/ml) were incubated with GM-CSF (12.5 ng/ml) for 20 minutes, TNF-α (10 ng/ml) for 20 minutes, and different concentrations of fMLP (A) or PMA (B) for different time periods. Cells were then lysed, and proteins from 4 × 105 cells were analyzed with SDS-PAGE and immunoblotting with anti–phospho-Ser345 antibody or anti-p47phox antibody. Data are representative of 3 independent experiments using cells from different donors.