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Trafficking of Vps10 in vps1 and vps8 cells. Pulse-chase experiments were used to analyze the stability of Vps10 in wild-type (L3852), vps8 (WLX16-1A), and vps1 (ACX58-3C) cells. Cells were radiolabeled at room temperature with Expre35S35S for 5 min and chased for various times. Vps10 was immunoprecipitated and analyzed by SDS-PAGE and fluorography. Newly synthesized Vps10 undergoes degradation in vps1 (arrow) but remains stable in vps8 and wild-type cells.
