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Functionality of the p53 transgene in aged mice. Young (6 months) and old (16 months) mice of the indicated genotypes were left untreated or exposed to 5 or 10 Gy whole-body γ-irradiation (γ-IR). At 3 h post-irradiation, the thymus was extracted and thymocytes were analysed by flow cytometry to measure their DNA content and evaluate the percentage of apoptosis. Data correspond to individual mice, except the rightmost columns, which correspond to the average±s.d. of three mice.
