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. 2006 Mar 31;7(5):506–511. doi: 10.1038/sj.embor.7400665

Figure 4.

Figure 4

Role of Wiskott–Aldrich syndrome protein in cell adhesion. (A) MEG-01 cells were transfected with calcium- and integrin-binding protein (CIB), FLAG-tagged calcyclin and Myc-tagged Wiskott–Aldrich syndrome protein (WASP)-dVCA (residues 1–435). Transfected cells were lysed and immunoblotted with anti-WASP antibody (lanes 1–4), anti-FLAG (anti-FLAG conjugated with horseradish peroxidase) and CIB antibodies (lanes 5–8). WASP was immunoprecipitated from cell lysates with anti-WASP antibody followed by immunoblotting for WASP (lanes 9–12) and CIB and FLAG–calcyclin (lanes 13–16). Myc-tagged WASP-dVCA was also immunoprecipitated with anti-Myc monoclonal antibody followed by immunoblotting with anti-Myc antibody (lanes 17,18) and anti-CIB antibody (lanes 19,20). (B) Cells transfected with calcyclin, CIB and WASP-dVCA were tested for adhesion to fibrinogen in the presence of GRGDSP or GRGESP peptide. Data are the means±s.e. of three experiments.