Figure 4.

Hof1p is cell cycle regulated and phosphorylated. (A) Regulation of Hof1p levels. Strain YEF2016 (HOF1-HA) was synchronized with α-factor and then released from the block. Samples were removed at 12-min intervals. (B) Hof1p-HA mobility changes upon treatment with phosphatase. Protein was isolated from strain YEF2016 at 72 and 84 min after release from α-factor arrest. T, total protein; IP, immunoprecipitated Hof1p-HA protein; IP + CIP, immunoprecipitated Hof1p-HA protein treated with calf intestinal alkaline phosphatase.