Table 2.
Primers used in this study
| Name | Sequence |
|---|---|
| PCR-mediated deletionsa | |
| HOF1-forward | TTGGAAAGTGTACTACTAATATTCAGAAAAAGGTGAAAGACGGATCCCCGGGTTAATTAA |
| HOF1-reverse | TCTTTTATCAGAAAACTAGTAAAATTGATATACATCGAGAGAATTCGAGCTCGTTTAAAC |
| BNR1-forward | GTGACACAAAAGCAGATAAAAAAATAGCACAATCATCAGCGGATTGTACTGAGAGTGCACC |
| BNR1-reverse | TTATATAAGCTCCACAACTACATAAAATACTAAGTCTTCACTGTGCGGTATTTCACACCG |
| Tagging with HA or GFPb | |
| HOF1-forward-2 | AATTCCCTATAATTTCATTCAGCTACTGCATCAAGGTCTTCGGATCCCCGGGTTAATTAA |
| Cloning of HOF1c | |
| HOF1-forward-B1 | GAACCTTTAGGATCCACTGAATTA |
| HOF1-reverse-S1 | CCTAACGTCGACAATTATCGTGATTATTGTGTC |
a
Underlined sequences are immediately upstream or downstream of the ORF to be deleted.
b
The underlined sequence is immediately upstream of the stop codon of HOF1.
c
BamHI and SalI sites are underlined.