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. 2000 Feb;11(2):613–626. doi: 10.1091/mbc.11.2.613

Figure 8.

Figure 8

VPS21 is epistatic to VPS27 for Vph1p trafficking. The production of Vph1p was under the control of the galactose-inducible GAL1 promoter in all strains. pep4-3 (NBY79), vps21-T39K pep4-3 (SGY46), vps27Δ pep4-3 (SGY77), and vps21-T39K vps27Δ pep4-3 (SGY78) strains were grown to midlog phase at 25°C, galactose was added, and the cells were incubated for 30 min at 37°C. Protein synthesis was terminated by the addition of cycloheximide, followed by incubation for an additional 15 min at 37°C before fixation. Vph1p and Vps10p were labeled with rabbit anti-Vph1p or rabbit anti-Vps10p antibodies, respectively, and Alexa 594–conjugated secondary antibody. DIC, differential interference contrast.