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. 2006 Jun 14;397(Pt 1):187–194. doi: 10.1042/BJ20051643

Figure 3. Levels of eIF2α(P) in different brain tissues prepared from mice subjected to SE.

Figure 3

(A) Lysates were prepared from the cortex (CTX), hippocampus (HPC), brain stem (BS) and whole brain (Total brain) from control animals (C) or mice after 30 min (30′), 2 h and 4 h of sustained SE. Equal amounts of each lysate were analysed by immunoblots using antibodies that specifically recognize the phosphorylated form of eIF2α or total eIF2α. (B) Quantification of eIF2α(P). Band intensities obtained from three independent experiments with four animals per group each were quantified by Kodak Digital Science software and normalized assuming a value of 1 to the ratio of eIF2α(P)/eIF2α obtained from control animals. Results are means±S.D.