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. 2006 Jun 14;397(Pt 1):39–45. doi: 10.1042/BJ20051957

Figure 4. Tiam1 signalling recruits the Arp2/3 complex to the cytoskeletal fraction.

Figure 4

(A) Tiam1 promotes Arp2/3 localization to the actin cytoskeleton fraction. MDCKII cells expressing either empty vector (E.V.) or HA-tagged Tiam1, ΔPHn3-Tiam1 or ΔDH-Tiam1 were fractionated in a Triton X-100 soluble (S fraction) and insoluble actin cytoskeleton fraction (I fraction). Fractions were either stained for Arp3 or p42 MAPK. The bar diagrams indicate the ratio of Arp3 or p42 MAPK of the insoluble (I) over the soluble (S) fraction. Total cell lysates were stained with an anti-HA antibody to detect the different Tiam1 mutants. Arp3 and p42 MAPK levels were quantified relative to E.V. transfected cells (bar diagram). Values (arbitrary units) are the means±S.E.M for three different experiments. WB, Western blot; WT, wild-type. (B) Tiam1 promotes Arp2/3 localization to cell–cell junctions. MDCKII cells transfected with E.V. or C1199-Tiam1 were immunostained for exogenous Tiam1 (green) and endogenous Arp3 (red). Scale bar, 10 μm.