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. 2006 Jun 16;103(26):10011–10016. doi: 10.1073/pnas.0602187103

Fig. 2.

Fig. 2.

M. smithii enhances B. thetaiotaomicron polyfructose-containing glycan degradation in the distal gut. Results of GeneChip analysis of RNA isolated from cecal contents of individual mice colonized with B. thetaiotaomicron ± M. smithii (n = 4–5 per group) are shown. (A) Unsupervised hierarchical clustering (dchip) of B. thetaiotaomicron (Bt) glycoside hydrolases (GH) and polysaccharide lysases (PL) that are up-regulated (13 genes) or down-regulated (57 genes) in the presence of M. smithii (Ms). Some GH/PL families represented in this data set are highlighted by using the classification scheme in CAZy (http://afmb.cnrs-mrs.fr/CAZY/acc.html; see Table 2 for a complete list). Each column represents data obtained from a cecal sample harvested from an individual mouse, whereas each row represents a B. thetaiotaomicron GH or PL gene. (B) B. thetaiotaomicron polyfructose degradation cluster induced in the presence of M. smithii (see Table 1 for fold changes defined by GeneChip). (C) Biochemical analysis of fructan and glucan levels in cecal contents (n = 5 mice per group; each sample assayed in duplicate; mean values ± SEM plotted). ∗, P < 0.05.