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. 2006 Jun 16;103(26):10011–10016. doi: 10.1073/pnas.0602187103

Fig. 3.

Fig. 3.

Biassociation with B. thetaiotaomicron and M. smithii increases B. thetaiotaomicron production of acetate and formate. (A) qRT-PCR analysis (boxed numbers) of the effects of M. smithii on expression of selected B. thetaiotaomicron genes encoding enzymes involved in fermentation of polyfructose-containing glycans: fructofuranosidases, BT1765/BT1759; fructokinase, BT1757; phosphofructokinase, BT0307; pyruvate:formate lyase, BT4738; acetate kinase, BT3963, methylmalonyl-CoA decarboxylase, BT1688; butyrate kinase, BT2552. Enzyme classification (EC) numbers are provided in parentheses. Dotted lines indicate multistep pathways. (Expression of fructofuranosidases, acetate kinase, puruvate:formate lyase, and butyrate kinase is constant if the colonization period is extended from 14 to 28 d; see Table 3.) (B) GC-MS analyses of cecal SCFAs (n = 5 per group; each sample assayed in duplicate; mean values ± SEM plotted; ∗, P < 0.05). (C) qRT-PCR study of the in vivo expression of M. smithii genes in a cluster (Lower) containing a formate transporter and dehydrogenase (fdhCAB) plus tungsten-containing formylmethanofuran dehydrogenase subunits (fwdEFDBAC) (n = 5 per group; each sample assayed in triplicate; mean values ± SEM plotted; ∗, P < 0.05).