Table 1.
Primary and secondary antisera.
| a. Primary Antisera | ||||
| Antigen | Host | Source | Working Dilution | |
| GFRα1 | Goat | R&D Systems (Minneapolis, MN, USA) | 1:200 | |
| GFRα2 | Goat | R&D Systems | 1:200 | |
| Synapsin | Rabbit | Biogenesis (Poole, UK) | 1:1000 | |
| Synaptophysin | Mouse | Dako (Botany, NSW, Australia) | 1:200 | |
| Tyrosine hydroxylase | Mouse | DiaSorin (Stillwater, MN, USA) | 1:2000 | |
| Vasoactive intestinal peptide | Rabbit | Dia Sorin | 1:2000 | |
| b. Secondary Antisera | ||||
| Antigen | Host | Conjugate | Source | Working Dilution |
| Goat IgG | Donkey | Cy3 | Jackson (West Grove, PA, USA) | 1:1000 |
| Mouse IgG | Horse | Biotin | Vectorlabs (Burlingame, CA, USA) | 1:200 |
| Rabbit IgG | Donkey | FITC | Jackson | 1:50 |