Fig. 7.
Relative fluorescence intensity changes of solutions of 2 (5.53 × 10−6 M) in Hepes buffer (pH 7.0) in the presence of various gangliosides, phospholipids, and other charged and neutral analytes. Ganglioside concentration was 0.5 mg/ml, ≈10−4 M each. Concentration of other analytes was 1.1 × 10−3 M. The standard deviation (n = 3) of the relative fluorescence intensity for each analyte ranges from 0.01 to 0.11. Proteins such as myelin and BSA were studied at 1 mg/ml concentrations. Asialo-GM1, asialoganglioside GM1; GM1, monosialoganglioside GM1; GD1a and GD1b, disialogangliosides; PI, l-α-phosphatidylinositol; PE, l-α-phosphatidylethanolamine; PS, l-α-phosphatidylserine; CMP-NANA, cytidine-5′-monophospho-N-acetylneuraminic acid.