Figure 1.

N73pep inhibits p115 binding to the Golgi apparatus. The N-terminal peptide of GM130 (N73pep) was microinjected together with cascade blue–conjugated BSA as an injection marker into the cytoplasm of NRK cells. After 1 h, the cells were fixed, permeabilized, and double labeled with a mAb to p115 and polyclonal antibodies to GM130 followed by fluorescently conjugated secondary antibodies (Texas red anti-mouse and FITC anti-rabbit antibodies). The injected cell is marked by an asterisk. Bar, 15 μm.