Figure 3.

Truncated GM130 replaces endogenous GM130 on the Golgi apparatus and prevents p115 binding. The N-terminally truncated Δ63 GM130 was transiently expressed in NRK cells by microinjection of the encoding plasmid. Cells were fixed and permeabilized by treatment with cold methanol and double labeled with monoclonal (B) or polyclonal (D and F) antibodies to GM130 and rabbit antibodies to the N-terminal domain of GM130 (A), a mAb to p115 (C), or a mAb to β-1,4 galactosyltransferase (GalT; E). Secondary antibodies were coupled to FITC (anti rabbit) or Texas red (anti mouse). Bar, 15 μm.