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. 2000 Feb;11(2):635–645. doi: 10.1091/mbc.11.2.635

Figure 6.

Figure 6

Increase in Golgi vesicles after replacing endogenous GM130 by the Δ63 GM130 mutant lacking the p115-binding site. Δ63 GM130 or fl GM130 was transiently expressed by microinjection of the cDNA into the nuclei of NRK cells. Protein A–gold was coinjected as a marker to identify microinjected cells (gold particles in the nuclei are indicated by arrows). After 3 h of expression, the cells were fixed and processed for electron microscopy. In cells expressing the Δ63 GM130 mutant, the number of vesicles located in the Golgi area increased and the length of the cisternae decreased (top). In contrast, overexpression of fl GM130 (bottom) had no effect on the morphology of the Golgi apparatus (see Figure 2, bottom). See Table 2 for quantitations. Bar, 1 μm.