Table 1.

Reduced p115 binding to the Golgi apparatus leads to an accumulation of vesicles in the Golgi region

Injection	Incubation time (h)	Number of vesicles per square meter of Golgi area		Increase in vesicle number (%)
		Injected cells	Uninjected cells
N73pep	1	31  ± 4.2	23  ± 3.4	35
N73pep	3	31  ± 5.4	22  ± 5.8	41
Control peptide	1	28  ± 5.1	26  ± 4.2	8
Protein A–gold	1	35  ± 5.2	32  ± 2.6	9

NRK cells were microinjected with N73pep or a control peptide (p115.4, residues 907–919 of p115) together with 10-nm protein A–gold as a marker to identify injected cells. The cells were incubated for 1 or 3 h, fixed, and processed for electron microscopy. The number of vesicles per square meter of Golgi area was determined for injected and uninjected cells as described in MATERIALS AND METHODS.