Figure 2.

The secretory pathway is required for proper Aip3p localization. The wild-type strain DBY2061 (A), sec4-8 strain DBY5894 (B), and sec17-1 strain DBY5856 (C) carrying the GFP-Aip3p–expressing Cen vector pDAb204, were shifted to 37°C for 90 min and examined by fluorescence microscopy. GFP fluorescence is shown on the left of each panel, and a DIC image of the same cells is shown on the right of each panel. GFP-Aip3p localization was quantified in several secretory pathway mutants after a shift to 37°C for 90 min (D). Individual cells were scored for the mislocalization of some of their pool of GFP-Aip3p, and the values reported are derived from counts of 200–350 cells.