Figure 8.

The addressing domain of Aip3p is conserved. The sequence alignment tool BLAST was used to align the protein sequences of AIP3 and an S. pombe gene of unknown function (FAT1, GenBank accession number Z97208; A). The GFP-Aip3p-encoding plasmid pDA278 and the GST-aip3-encoding plasmid pHJ29 were transformed into strain FY86, and the strain was grown either continuously in glucose-containing medium (GLU) or shifted out of raffinose-containing medium into galactose-containing medium for 4 h (GAL), and the percentage of cells able to correctly localize some of their pool of GFP-Aip3p was scored (B). Strain FY86 was transformed with either the GST-expressing plasmid pEG(KT) (Vector) or the GST-aip3p–expressing plasmid pHJ29 (GST-aip3p), and the transformants were grown on galactose-containing plates at 25 or 37°C (C).