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The N terminus of Fat1p can function in the secretory pathway-mediated localization pathway. Wild-type strain FY23×86 was transformed with plasmid pHJ36 encoding a fusion of GFP to amino acids 135–478 of Aip3p (A) and plasmid pHJ37 encoding a fusion of GFP to the N-terminal 175 amino acids of Fat1p, followed by amino acids 135–478 of Aip3p (B). The transformants were examined by fluoresence microscopy (left) and DIC microscopy (right).
