Table 2.
Plasmids used in this study
| Plasmid | Markera | Reference of source |
|---|---|---|
| Phosphatase plasmid | ||
| pXZ209 | HIS3 CEN PTP3 | This study |
| pXZ210 | HIS3 CEN ptp3C814G | This study |
| pXZ223 | HIS3 CEN PTP2 | This study |
| pXZ134 | URA3 2μ PADH1-GST | Zhan and Guan, 1999 |
| pXZ110 | URA3 2μ PADH1-GST-PTP1 | Zhan and Guan, 1999 |
| pXZ113 | URA3 2μ PADH1-GST-PTP2 | Zhan and Guan, 1999 |
| pXZ123 | URA3 2μ PADH1-GST-PTP3 | Zhan and Guan, 1999 |
| pXZ136 | URA3 2μ PADH1-GST-ptp3C814G | Zhan and Guan, 1999 |
| Kinase plasmid | ||
| Flag-MCK1 | TRP1 2μ PMCK1-FLAG-MCK1 | This study |
| pKB166 | URA3 2μ PRIM11-HA-RIM11 | Bowdish et al., 1994 |
| pKB199 | URA3 2μ PRIM11-HA-rim11K68A | Bowdish et al., 1994 |
| pKB201 | URA3 2μ PRIM11-HA-rim11Y199F | Bowdish et al., 1994 |
| pXZ356 | URA3 2μ PRIM11-HA-rim11Y199E | This study |
| pXZ352 | HIS3 CEN PRIM11-HA-RIM11 | This study |
| pXZ353 | HIS3 CEN PRIM11-HA-rim11K68A | This study |
| pXZ354 | HIS3 CEN PRIM11-HA-rim11Y199F | This study |
| pXZ355 | HIS3 CEN PRIM11-HA-rim11Y199E | This study |
| pXZ118 | GST-HA-RIM11 in E. coli expression vector | This study |
| pXZ119 | GST-HA-rim11K68A in E. coli expression vector | This study |
| pXZ120 | GST-HA-rim11Y199F in E. coli expression vector | This study |
a
PADH1, PMCK1, and PRIM11, are ADH1, MCK1, and RIM11 promoters, respectively.