Figure 3.

Fibronectin-coated (A) and VCAM-1-coated (B) bead binding of J-β2.7 cells and different clones of J-β2.7 cells expressing wt LFA-1 or ΔI-LFA-1. Cells were adhered to plastic with an anti-CD5 mAb and incubated with fibronectin- or VCAM-1-coated beads for 90 min at 37°C before washing off unbound beads. Bound cells and beads were fixed in 1% formaldehyde. Quantification was carried out by counting cells and beads per high-power field. Data are represented as beads per 100 cells from the mean of five high-power fields ± SD. Data are representative of two experiments with identical results.