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Expression of α4β1 and α5β1 on untransfected J-β2.7 cells and on different J-β2.7 clones expressing wt LFA-1 or ΔI-LFA-1. Cells were stained on ice with the mAbs SAM-1 (anti-α5) or HP1/2 (anti-α4) followed by FITC-conjugated goat anti-mouse IgG and analysis by flow cytometry. Dotted lines, negative control; filled histograms, mAb SAM-1; open histograms, mAb HP1/2.
