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. 2000 Feb;11(2):691–702. doi: 10.1091/mbc.11.2.691

Figure 1.

Figure 1

Western analysis of selected two-hybrid constructs. (A) Protein expression of SMY1 fragments inserted into pGAD-C(x). The blot was probed with affinity-purified polyclonal anti-Smy1p. The ∼70-kDa band is endogenous Smy1p. (B) Protein expression of MYO2 and myo2–2 fragments. M2, M2–2, and M3 fragments are inserted into pGAD-C(x), whereas M73 and M76 are in pBTM116. The ∼180-kDa band is endogenous Myo2p. Because of their strong expression, a shorter time exposure (25 s instead of 2 min) is shown for M73 and M76. Expression levels and constructs are described in MATERIALS AND METHODS. Constructs also are described in Table 2 and illustrated in Figure 2.