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. 2000 Feb;11(2):691–702. doi: 10.1091/mbc.11.2.691

Figure 3.

Figure 3

Domain mapping of the Smy1p–Myo2p interaction by two-hybrid analysis. (A) SMY1 fragments were inserted into pGAD-C(x) and tested for two-hybrid interaction with pBTM-MYO2. (B) Fragments of MYO2 and myo2–2 inserted into pGAD-C(x), Myo2R, M1, M2, M3, M4, and M2–2 were tested for interaction with pBTM-SMY1(D1). M73, M76, and M11 were constructed in pBTM116 and tested for interaction with pGAD-SMY1. M3 and M4 fragments were also inserted into pBTM-C1 and pBTM116, respectively. The asterisk indicates the G1248 to D1248 mutation of myo2–2p (Catlett and Weisman, 1998).