Table 1.
Yeast strains used in this study
| Strain | Relevant genotypea | Source |
|---|---|---|
| Y190 | MATa gal4 gal80 leu2-3,112 trp1-901 his3 ade2-101 URA∷GAL1b-lacZ LYS2∷GAL1-HIS3 | S. Elledge |
| L40 | MATa leu2 trp1 his3 LYS2∷lexA-HIS3 URA3∷lexAc-lacZ | R. Sternglanz |
| SLY88 | MATa myo2-66 ura3-52 leu2-3,112 trp1-Δ1 his3 his6 | |
| MATα myo2-66 ura3-52 leu2-3,112 TRP1 HIS3 HIS6 | Lillie and Brown (1994) | |
| SLY86 | MATa smy1-Δ2∷LEU2 ura3-52 leu2-3,112 trp1-289 his4 | |
| MATα smy1-Δ2∷LEU2 ura3-52 leu2-3,112 TRP1 his4 | Lillie and Brown (1994) | |
| LWY2599 | MATa myo2-2 his3Δ-200 lys2-801 leu2 trp1-Δ901 suc2-Δ9 ade8∷HIS3 | L. Weisman |
| LWY7213 | MATa MYO2 his3Δ-200 lys2-801 leu2 trp1-Δ901 suc2-Δ9 ade8∷HIS3 | L. Weisman |
a
Some strains may carry additional mutations; most or all are S288C derivatives and therefore gal2.
b
Contains 4 Gal4 DNA-binding sites within the GAL1 promoter (Clontech, Palo Alto, CA).
c
Contains 8 LexA DNA-binding sites within the lexA promoter (Dagher and Filhol-Cochet, 1997).