Figure 5.

Effects of D-4F Treatments on Plasma MPO Concentrations, MPO association with apoA-I and 3NT formation in apoA-I in Plasma of Hypercholesterolemic Ldlr ^−/− mice. (A) Plasma MPO concentrations in WD-fed Ldlr ^−/− mice ± D-4F (ip) were measured by ELISA (HK210, Cell Sciences, Canton, MA). D-4F has no effect on total MPO concentrations in Ldlr ^−/− mice fed WD. (B) Autoradiograms of western blot analysis for MPO association with and 3NT formation in apoA-I that was immunoprecipitiated from hypercholesterolemia Ldlr ^−/− mice treated with PBS or D-4F in PBS by ip. Autoradiograms showing D-4F decreases MPO association with apoA-I, with a concurrent decrease in 3NT formation in apoA-I in the plasma of the hypercholesterolemic Ldlr ^−/− mice. (C) Bar graph showing means ± SEMs of relative density of MPO association and 3NT formation in the WD-fed Ldlr ^−/− mice ± D-4F treatments (n=5). D-4F decreases MPO association with apoA-I and 3NT residues in apoA-I in WD-fed Ldlr ^−/− mice. (D) Autoradiograms of western blots for MPO and 3NT in apoA-I of apoA-I immunoprecipitates from plasma from C57BL/6 mice. Plasma was spiked with D-4F (10 μg/mL, final concentration) and incubated overnight. ApoA-I was immunoprecipitated as described in Methods. Western blot analysis for MPO and apoA-I was as above. (*=p<0.05, ** = p<0.02).