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. Author manuscript; available in PMC: 2006 Jun 20.
Published in final edited form as: J Med Chem Biol Radiol Def. 2005 Jul 1;3:nihms5095. doi: 10.1901/jaba.2005.3-nihms5095

Figure 1.

Figure 1

Purification of BChE by ion exchange at pH 4.0 The BChE in 62 L of dialyzed human plasma was bound to 4 L of Q-Sepharose Fast Flow (QFF) at pH 4.0 in a batch process. The QFF was packed into a 7 L glass column and washed with 200 L of endotoxin-free 20 mM sodium acetate, 1 mM EDTA pH 4.0, at 4°C. The graph shows elution of BChE from QFF with 0.05 M NaCl in pH 4.0 buffer at a flow rate of 1.7 L per hour. The BChE in fraction #1 was purified 1200-fold.