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Nuclear localization of NTF2 is independent of Ran binding. Ran binding mutants of NTF2, E42K (a) and H124Δ (c), which eliminate or significantly reduce binding to Ran, respectively, were biotinylated and coinjected with FITC-dextran as an injection marker (b and d) into the cytoplasm of HeLa cells. Cells were incubated at 37°C for 30 min aftet injection, fixed, permeabilized, and stained with antibiotin mAb and rhodamine anti-mouse IgG.
