Figure 7.

Preincubation of cytosol with anti-NTF2 mAbs inhibits Ran nuclear import in permeabilized cells. (a–k) Adherent HeLa cells grown on glass coverslips were permeabilized with digitonin and incubated with an energy-regenerating system for 15 min at room temperature. Cells were incubated without exogenous HeLa cytosol (a and c), with exogenous HeLa cytosol (b and d), or with exogenous HeLa cytosol that had been preincubated on ice for 15 min with 0.5 mg/ml WGA (e), 1 mg/ml anti-NPC mAb QE5 (f), or 1 mg/ml anti-NTF2 mAb (g–k). Cells were stained for Ran with either anti-Ran mAb and rhodamine anti-mouse IgG (a and b) or anti-Ran polyclonal antibodies and FITC anti-rabbit IgG (c–k).