Skip to main content
. 2006 Jun 12;103(25):9476–9481. doi: 10.1073/pnas.0603694103

Table 3.

Holoenzyme synthase activity of hMSR

apoMS treatment MS activity, nmol/min
MeCbl
    w/hMSRI-H and NADPH 0.83
    w/hMSRI-H 0.62
    w/NADPH 0.39
    w/o hMSRI-H and NADPH 0.35
aqCbl
    w/hMSRI-H and NADPH 0.24
    w/hMSRI-H 0.11
    w/NADPH 0.02
    w/o hMSRI-H and NADPH 0.01
No Cbl
    w/hMSRI-H and NADPH 0.06
    w/o hMSRI-H and NADPH <0.01

A crude extract from Sf9 cells infected with recombinant baculovirus was used as the source of human apoMS. A mixture (total volume was 200 μl) that contained 150 μl of crude extract (2.2 mg/ml protein) and the components indicated (w/, with; w/o, without) was preincubated for 15 min at 37°C prior to measurement of MS activity by the aqCbl/DTT assay. The concentrations of the components were 50 μM MeCbl or aqCbl, 200 nM hMSRI-H, and 200 μM NADPH.