Fig. 2.

Initial maximum intensity against [Ca²⁺] of mutant and WT aequorins. (A) Bright and SloDK mutants. The plot of the L¹⁷⁰I Bright mutant (not shown) was almost identical to that of Q¹⁶⁸R. (B) Double EF-hand mutants. EF1⁺2⁻3⁻, EF1⁻2⁺3⁻, and EF1⁻2⁻3⁺ correspond to the E¹²⁸G and D¹⁵³G, E³⁵G and D¹⁵³G, and E³⁵G and D¹²⁸G double mutants, respectively.