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Pulse–chase analysis of PfiTPT and PfoTPT processing. (A) A 57-kDa PfiTPT precursor (arrowhead) is depleted during a 2-h chase, whereas a 43-kDa mature form of PfiTPT (arrow) increases in abundance. The 43-kDa immunoprecipitated protein was N-terminally sequenced and lacks the first 140 aa encoded by the PfiTPT gene. (B) The 34-kDa PfoTPT protein does not undergo any size change during a 2-h chase, suggesting no processing.
