Fig. 1.

Chloroplast localization of DSP4-GFP. (A) Structural domains of DSP4 protein: green, predicted transit peptide; red, catalytic domain; blue – starch binding domain. (B–F) Chloroplast localization of the DSP4 protein visualized by GFP reporter protein. (B) GFP control in leaves. (C and D) Scans in the red and green channels for chlorophyll fluorescence (C) and DSP4-GFP (D). (E and F) Overlaid images showing DSP4-GFP localization in the chloroplasts at two different magnifications. Green is the true GFP fluorescence after linear unmixing (See Materials and Methods), red is chloroplast autofluorescence. (Scale bars, 5 μm in B and C and 20 μm in E and F.) (G) Emission spectrum across the green image area after linear unmixing. The major GFP emission peak is at 543 nm.