Figure 4.

Palmitoylation of apoB-29 is required for assembly of neutral lipids into the hydrophobic core of the lipoprotein particle. (A) TLC analysis of lipid content corresponding to individual density gradient fractions 1–7 (depicted in Figure 3) is shown for WT apoB-29 (WT) or nonpalmitoylated mutant apoB-29 (Mut). (B) An aliquot of the lipid extract corresponding to one-tenth of pooled gradient fractions 11–20 isolated from McArdle-RH7777 cells secreting either WT apoB-29 (WT) or Cys1085Ser mutant apoB-29 (Mut). Positions of lipid standards are indicated on the left for CE, TG, free fatty acid (FFA), phosphatidylethanolamine (PE), and phosphatidylcholine (PC). The TLC plate was exposed to film for 16 h.