TABLE 1.
Strains used in this study
| Strain | Genotype or description | Reference, source, and/or method of construction |
|---|---|---|
| E. coli | ||
| DH10B | F−mrcA Δ(mrr hsdRMS mcrBC) φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara leu) 7697 araD139 galU galK nupG rpsL | GIBCO BRL |
| S17-1 | M294::RP4-2 (Tet::Mu) (Kan::Tn7) | 38 |
| C. crescentus | ||
| LS1218 | NA1000 ΔfliF | 12 |
| LS1528 | ΔfliF::fliF (wild-type control for all strains carrying fliF derivatives) | LS1218::pUJ70; 12 |
| LS1944 | ΔfliF/pfliFΔ1360-1425 (FliFΔ454-475 Δ1) | LS1218/pUJ99; 12 |
| NA1000 | Synchronizable holdfast mutant derivative of wild-type strain CB15 | 3 |
| UJ355 | ΔfliF::fliFΔ1579-1593 (FliFΔ527-531 ΔI) | LS1218::pBG1 |
| UJ356 | ΔfliF::fliFΔ1594-1608 (FliFΔ532-536 ΔII) | LS1218::pBG2 |
| UJ357 | ΔfliF::fliFΔ1609-1623 (FliFΔ537-541 ΔIII) | LS1218::pBG3 |
| UJ358 | ΔfliF::fliFΔ1624-1638 (FliFΔ542-546 ΔIV) | LS1218::pBG4 |
| UJ359 | ΔfliF::fliFΔ1639-1650 (FliFΔ547-550 ΔV) | LS1218::pBG5 |
| UJ360 | ΔfliF::fliFΔ1651-1662 (FliFΔ551-554 ΔVI) | LS1218::pBG6 |
| UJ434 | ΔfliF::fliFΔ1579-1656 (FliFΔ527-552 Δ5) | LS1218::pBG7 |
| UJ553 | ΔfliF::fliFΔ1594-1656 (FliFΔ532-552 Δ6) | LS1218::pBG8 |
| UJ554 | ΔfliF::fliFΔ1609-1656 (FliFΔ537-552 Δ7) | LS1218::pBG9 |
| UJ555 | ΔfliF::fliFΔ1624-1656 (FliFΔ542-552 Δ8) | LS1218::pBG10 |
| UJ556 | ΔfliF::fliFΔ1639-1656 (FliFΔ547-552 Δ9) | LS1218::pBG11 |
| UJ857 | ΔfliF::fliFΔ1579-1638 (FliFΔ527-546 Δ10) | LS1218::pSG1 |
| UJ858 | ΔfliF::fliFΔ1579-1623 (FliFΔ527-541 Δ11) | LS1218::pSG2 |
| UJ859 | ΔfliF::fliFΔ1579-1608 (FliFΔ527-536 Δ12) | LS1218::pSG3 |
| UJ881 | Mot− suppressor of UJ355 | This study |
| UJ882 | Mot− suppressor of UJ355 | This study |
| UJ883 | Mot− suppressor of UJ355 | This study |
| UJ887 | Mot− suppressor of UJ356 | This study |
| UJ970 | ΔfliF::fliFΔ1594-1638 (FliFΔ532-546 Δ13) | LS1218::pSG4 |
| UJ971 | ΔfliF::fliFΔ1594-1608, 1624-1638 (FliFΔ532-536, 542-546 Δ14) | LS1218::pSG5 |
| UJ974 | ΔfliF::fliF-S3 (FliFV543D A544R I545E L546K) | LS1218::pSG8 |
| UJ975 | ΔfliF::fliF-S4 (FliFS532R F534K V535D) | LS1218::pSG9 |
| UJ1077 | ΔfliF::fliFΔ1426-1488 (FliFΔ476-496 Δ15) | LS1218::pSG10 |
| UJ1078 | ΔfliF::fliFΔ1489-1536 (FliFΔ497-512 Δ16) | LS1218::pSG11 |
| UJ1079 | ΔfliF::fliFΔ1537-1578 (FliFΔ513-526 Δ17) | LS1218::pSG12 |
| UJ1236 | NA1000 Δ(fliF-fliG) | This study |
| UJ1471 | Mot− suppressor of UJ858 | This study |
| UJ1479 | Mot− suppressor of UJ975 | This study |
| UJ1480 | Mot− suppressor of UJ975 | This study |
| UJ1482 | Mot− suppressor of UJ975 | This study |
| UJ1512 | Δ(fliF-fliG)/p(fliF-fliG)UJ881 | UJ1236/pBG38 |
| UJ1513 | Δ(fliF-fliG)/p(fliF-fliG)UJ882 | UJ1236/pBG39 |
| UJ1514 | Δ(fliF-fliG)/p(fliF-fliG)UJ883 | UJ1236/pBG40 |
| UJ1518 | Δ(fliF-fliG)/p(fliF-fliG)UJ887 | UJ1236/pBG44 |
| UJ1522 | Δ(fliF-fliG)/p(fliF-fliG)LS1528 | UJ1236/pBG60 |
| UJ1523 | Δ(fliF-fliG)/p(fliF-fliG)UJ1471 | UJ1236/pBG48 |
| UJ1531 | Δ(fliF-fliG)/p(fliF-fliG)UJ1479 | UJ1236/pBG56 |
| UJ1532 | Δ(fliF-fliG)/p(fliF-fliG)UJ1480 | UJ1236/pBG57 |
| UJ1534 | Δ(fliF-fliG)/p(fliF-fliG)UJ1482 | UJ1236/pBG59 |