TABLE 2.
Plasmids used in this study
| Plasmid | Description | Reference or source |
|---|---|---|
| pBG1 | fliFΔ1579-1593 (ΔI) in pBGS18T as BamHI-EcoRI fragment | This study |
| pBG2 | fliFΔ1594-1608 (ΔII) in pBGS18T as BamHI-EcoRI fragment | This study |
| pBG3 | fliFΔ1609-1623 (ΔIII) in pBGS18T as BamHI-EcoRI fragment | This study |
| pBG4 | fliFΔ1624-1638 (ΔIV) in pBGS18T as BamHI-EcoRI fragment | This study |
| pBG5 | fliFΔ1639-1650 (ΔV) in pBGS18T as BamHI-EcoRI fragment | This study |
| pBG6 | fliFΔ1651-1662 (ΔVI) in pBGS18T as BamHI-EcoRI fragment | This study |
| pBG7 | fliFΔ1579-1656 (Δ5) in pBGS18T as BamHI-EcoRI fragment | This study |
| pBG8 | fliFΔ1594-1656 (Δ6) in pBGS18T as BamHI-EcoRI fragment | This study |
| pBG9 | fliFΔ1609-1656 (Δ7) in pBGS18T as BamHI-EcoRI fragment | This study |
| pBG10 | fliFΔ1624-1656 (Δ8) in pBGS18T as BamHI-EcoRI fragment | This study |
| pBG11 | fliFΔ1639-1656 (Δ9) in pBGS18T as BamHI-EcoRI fragment | This study |
| pBG22 | Sequence flanking Δ(fliF-fliG) in pNPTS128 as BamHI-EcoRI fragment | This study |
| pBG38 | (fliF-fliG)UJ881 in pMR10 as BamHI-EcoRV fragment | This study |
| pBG39 | (fliF-fliG)UJ882 in pMR10 as BamHI-EcoRV fragment | This study |
| pBG40 | (fliF-fliG)UJ883 in pMR10 as BamHI-EcoRV fragment | This study |
| pBG44 | (fliF-fliG)UJ887 in pMR10 as BamHI-EcoRV fragment | This study |
| pBG48 | (fliF-fliG)UJ1471 in pMR10 as BamHI-EcoRV fragment | This study |
| pBG56 | (fliF-fliG)UJ1479 in pMR10 as BamHI-EcoRV fragment | This study |
| pBG57 | (fliF-fliG)UJ1480 in pMR10 as BamHI-EcoRV fragment | This study |
| pBG59 | (fliF-fliG)UJ1482 in pMR10 as BamHI-EcoRV fragment | This study |
| pBG60 | (fliF-fliG)LS1528 in pMR10 as BamHI-EcoRV fragment | This study |
| pBGS18T | Kanr derivative of pUC18 with oriT (suicide vector in C. crescentus) | M. R. K. Alley |
| pCM4 | flgF promoter in pRKlac290 as HindIII/EcoRI fragment | 28 |
| pMR10 | Kanr low copy number, broad-host-range vector | C. Mohr and R. Roberts |
| pNPTS128 | Kanr derivative of pLITMUS28 with sacB and oriT | M. R. K. Alley |
| pRKlac290 | RK2-based lacZ transcriptional fusion vector (Tetr) | 7 |
| pSG1 | fliFΔ1579-1638 (Δ10) in pBGS18T as BamHI-EcoRI fragment | This study |
| pSG2 | fliFΔ1579-1623 (Δ11) in pBGS18T as BamHI-EcoRI fragment | This study |
| pSG3 | fliFΔ1579-1608 (Δ12) in pBGS18T as BamHI-EcoRI fragment | This study |
| pSG4 | fliFΔ1594-1638 (Δ13) in pBGS18T as BamHI-EcoRI fragment | This study |
| pSG5 | fliFΔ(1594-1608 1624-1638) (Δ14) in pBGS18T as BamHI-EcoRI fragment | This study |
| pSG8 | fliF S3 in pBGS18T as BamHI-EcoRI fragment | This study |
| pSG9 | fliF S4 in pBGS18T as BamHI-EcoRI fragment | This study |
| pSG10 | fliFΔ1426-1488 (Δ15) in pBGS18T as BamHI-EcoRI fragment | This study |
| pSG11 | fliFΔ1489-1536 (Δ16) in pBGS18T as BamHI-EcoRI fragment | This study |
| pSG12 | fliFΔ1537-1578 (Δ17) in pBGS18T as BamHI-EcoRI fragment | This study |
| pUJ70 | fliF in pBGS18T as BamHI-EcoRI fragment | 12 |